TY  - JOUR
TI  - Immunoprecipitation of Cell Surface Proteins from Gram-negative Bacteria
AU  - da Cunha, Carlos Eduardo Pouey
AU  - Newcombe, Jane 
AU  - Dellagostin, Odir Antonio
AU  - McFadden, Johnjoe 
VL  - 7
IS  - 9
PY  - 2017
DA  - 2017/05/05
SP  - e2250
C1  - Bio-protocol 2017;7:e2250
DO  - 10.21769/BioProtoc.2250
UR  - https://doi.org/10.21769/BioProtoc.2250
AB  - The meningococcus (Neisseria meningitidis) remains an important threat to human health	worldwide. This Gram-negative bacterium causes elevated disabilities and mortality in infected	individuals. Despite several available vaccines, currently there is no universal vaccine against all	circulating meningococcal strains (Vogel et al., 2013). Herein, we describe a new protocol that is	capable of identifying only cell surface exposed proteins that play a role in immunity, providing this	research field with a more straightforward approach to identify novel vaccine targets. Even though N. meningitidis is used as a model in the protocol herein described, this protocol can be used for any	Gram-negative bacteria provided modifications and optimizations are carried out to adapt it to different	bacterial and disease characteristics (e.g., membrane fragility, growth methods, serum antibody levels,	etc.).
KW  - Gram-negative
KW  - Immunoproteome
KW  - Immunoprecipitation
KW  - Cell surface antigen
KW  - Outer	membrane protein
KW  - Exposed antigen
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False