TY  - JOUR
TI  - Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes
AU  - Doumane, Mehdi 
AU  - Lionnet, Claire 
AU  - Bayle, Vincent 
AU  - Jaillais, Yvon  
AU  - Caillaud, Marie-Cécile 
VL  - 7
IS  - 8
PY  - 2017
DA  - 2017/04/20
SP  - e2245
C1  - Bio-protocol 2017;7:e2245
DO  - 10.21769/BioProtoc.2245
UR  - https://doi.org/10.21769/BioProtoc.2245
AB  - Here we describe a protocol that enables to automatically perform time-lapse imaging of growing root tips for several hours. Plants roots expressing fluorescent proteins or stained with dyes are imaged while they grow using automatic movement of the microscope stage that compensates for root growth and allows to follow a given region of the root over time. The protocol makes possible the image acquisition of multiple growing root tips, therefore increasing the number of recorded mitotic events in a given experiment. The protocol also allows the visualization of more than one fluorescent protein or dye simultaneously, using multiple channel acquisition. We particularly focus on imaging of cytokinesis in Arabidopsis root tip meristem, but this protocol is also suitable to follow root hair growth, pollen tube growth, and other regions of root over time, in various plant species. It may as well be amenable to automatically track non-plant structures with an apical growth.
KW  - Cell division
KW  - Mitosis
KW  - Cytokinesis
KW  - Root
KW  - Microscopy
KW  - Tracking
KW  - Arabidopsis
KW  - Phosphoinositide
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False