TY  - JOUR
TI  - Preparation of Primary Astrocyte Culture Derived from Human Glioblastoma Multiforme Specimen
AU  - Hashemi, Mansoureh 
AU  - Hadjighassem, Mahmoudreza  
VL  - 7
IS  - 8
PY  - 2017
DA  - 2017/04/20
SP  - e2241
C1  - Bio-protocol 2017;7:e2241
DO  - 10.21769/BioProtoc.2241
UR  - https://doi.org/10.21769/BioProtoc.2241
AB  - Glioblastoma multiforme (GBM) is a grade 4 astrocytoma tumor in central nervous system. Astrocytes can be isolated from human GBM. Study of astrocytes can provide insights about the formation, progression and recurrence of glioblastoma. For using isolated astrocytes, new studies can be designed in the fields of pharmacology, neuroscience and neurosurgery for glioblastoma treatment. This protocol describes the details for preparing high purity primary astrocytes from human GBM. Tumor tissue is disrupted using mechanical dissociation and chemical digestion in this protocol. 2 weeks after plating the cell suspension in culture, primary astrocytes are available for further subculturing and immunocytochemistry of S100-beta antigen.
KW  - Glioblastoma multiforme
KW  - Astrocyte
KW  - S100-beta antigen
KW  - Primary cell culture
KW  - Malignant brain tumor
KW  - Astrocytoma tumor
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False