TY  - JOUR
TI  - Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells
AU  - Domanski, Michal 
AU  - LaCava, John 
VL  - 7
IS  - 8
PY  - 2017
DA  - 2017/04/20
SP  - e2238
C1  - Bio-protocol 2017;7:e2238
DO  - 10.21769/BioProtoc.2238
UR  - https://doi.org/10.21769/BioProtoc.2238
AB  - The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert et al., 2016]). The following protocol describes an approach to purify RNA exosome complexes from HEK-293 cells, making use of inducible ectopic expression, affinity capture, and rate-zonal centrifugation. The obtained RNA exosomes have been used successfully for proteomic, structural, and enzymatic studies (Domanski et al., 2016).
KW  - RNA exosome
KW  - EXOSC10
KW  - Cryomilling
KW  - HEK-293 suspension culture
KW  - Affinity capture
KW  - Rate-zonal centrifugation
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False