TY  - JOUR
TI  - Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition
AU  - Carnachan, Susan M.
AU  - Hinkley, Simon F. R.
VL  - 7
IS  - 7
PY  - 2017
DA  - 2017/04/05
SP  - e2197
C1  - Bio-protocol 2017;7:e2197
DO  - 10.21769/BioProtoc.2197
UR  - https://doi.org/10.21769/BioProtoc.2197
AB  - Heparan sulfate (HS) is purified from complex matrices and often not fully characterised to validate its assignment. The characterisation of heparins and heparan sulfates through enzymatic depolymerisation and subsequent strong anion-exchange high performance liquid chromatography (SAX-HPLC) analysis and quantitation of the resulting disaccharides is a critical tool for assessing the structural composition of this class of compound. This protocol details a methodology to reproducibly determine the disaccharide composition of heparan sulfate by enzymatic depolymerisation and SAX-HPLC analysis. A complementary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017).
KW  - Heparan sulfate
KW  - Glycosaminoglycan’s
KW  - Chemical characterization
KW  - Enzymatic depolymerisation
KW  - HPLC
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False