TY  - JOUR
TI  - Polysome Analysis
AU  - Poria, Dipak  Kumar 
AU  - Ray, Partho  Sarothi 
VL  - 7
IS  - 6
PY  - 2017
DA  - 2017/03/20
SP  - e2192
C1  - Bio-protocol 2017;7:e2192
DO  - 10.21769/BioProtoc.2192
UR  - https://doi.org/10.21769/BioProtoc.2192
AB  - Polysome analysis is a method to separate mRNAs from a cell into actively translating and non-translating fractions depending on their association with polysomes. By this protocol, cell lysates are fractionated by sucrose density gradient ultracentrifugation. Free mRNA fraction and various ribosomal fractions, such as 40S, 60S, monosomes and polysomes are collected by fractionation. Association of particular mRNAs with these fractions is detected by reverse transcription – PCR to investigate the translational state of the mRNA.
KW  - Translation
KW  - Ribosome fractionation
KW  - Polysome
KW  - mRNA
KW  - Sucrose density gradient
KW  - Ultracentrifugation
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False