TY  - JOUR
TI  - Measurement of Dipeptidylpeptidase Activity in vitro and in vivo
AU  - Barreira da Silva, Rosa  
AU  - Ingersoll, Molly  A. 
AU  - Albert, Matthew  L. 
VL  - 7
IS  - 6
PY  - 2017
DA  - 2017/03/20
SP  - e2184
C1  - Bio-protocol 2017;7:e2184
DO  - 10.21769/BioProtoc.2184
UR  - https://doi.org/10.21769/BioProtoc.2184
AB  - Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space. DPP4 is found at the surface of many hematopoietic and non-hematopoietic cells and is also present in many biological fluids in a bioactive soluble form. DPP4 expression is modulated by inflammation, and measurements of its activity have been used as biomarker for disease. Here, we describe a method to evaluate the enzymatic activity of DPP4 in vitro and in vivo.
KW  - Dipeptidylpeptidase 4
KW  - Enzyme
KW  - Tumor
KW  - Plasma
KW  - DPP4 inhibitor
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False