TY  - JOUR
TI  - In Gel Kinase Assay
AU  - Pizzio, Gaston A.
AU  - Rodruiguez, Pedro Luis
VL  - 7
IS  - 5
PY  - 2017
DA  - 2017/03/05
SP  - e2170
C1  - Bio-protocol 2017;7:e2170
DO  - 10.21769/BioProtoc.2170
UR  - https://doi.org/10.21769/BioProtoc.2170
AB  - Proper spatiotemporal regulation of protein phosphorylation in cells and tissues is required for normal development and homeostasis. We present the protocol ‘In Gel Kinase Assay’, which is useful for protein kinase activity measurements from crude protein extracts. We have successfully used ‘In Gel Kinase Assay’ protocol to show that the Arabidopsis thaliana sextuple mutant in the PYRABACTIN RESISTANCE1/PYR1-LIKE/REGULATORY COMPONENTS OF ABA RECEPTORS (PYR/PYL/RCAR-ABA receptors; line pyr/pyl112458) is impaired in ABA-mediated activation of SnRK2.2, SnRK2.3 and OST1/SnRK2.6, as much as the triple mutant snrk2.2/2.3/2.6 (Gonzalez-Guzman et al., 2012).
KW  - Kinases
KW  - Phosphorylation
KW  - in vivo
KW  - Enzymatic activity
KW  - SnRKs
KW  - ABA
KW  - Arabidopsis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False