TY  - JOUR
TI  - Thinned-skulled Cranial Window Preparation (Mice)
AU  - Zhang, Lifeng  
AU  - Liang, Bo  
AU  - Li, Yun  
AU  - Lin, Da-Ting  
VL  - 7
IS  - 5
PY  - 2017
DA  - 2017/03/05
SP  - e2158
C1  - Bio-protocol 2017;7:e2158
DO  - 10.21769/BioProtoc.2158
UR  - https://doi.org/10.21769/BioProtoc.2158
AB  - Imaging structural plasticity or activity of neurons in the brain circuit will facilitate understanding the neural mechanisms underlying animal behavior. Here we describe a modified procedure, the polished and reinforced thinned-skull cranial window preparation, by which we can image dendrites and spines in mouse layer I cortex for weeks (Zhang et al., 2016). By this method, we also imaged the glioma initiation in the mouse cortex for two weeks in previous work (Zhang et al., 2012), which included the photographs and video for reference.
KW  - Neuroscience
KW  - Mouse brain
KW  - in vivo imaging
KW  - Cranial window
KW  - Structural plasticity
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False