TY  - JOUR
TI  - Automatic Quantification of the Number of Intracellular  Compartments in Arabidopsis thaliana Root Cells
AU  -  Bayle, Vincent 
AU  - Platre , Matthieu  Pierre 
AU  - Jaillais, Yvon  
VL  - 7
IS  - 4
PY  - 2017
DA  - 2017/02/20
SP  - e2145
C1  - Bio-protocol 2017;7:e2145
DO  - 10.21769/BioProtoc.2145
UR  - https://doi.org/10.21769/BioProtoc.2145
AB  - In the era of quantitative biology, it is increasingly required to quantify confocal microscopy images. If possible, quantification should be performed in an automatic way, in order to avoid bias from the experimenter, to allow the quantification of a large number of samples, and to increase reproducibility between laboratories. In this protocol, we describe procedures for automatic counting of the number of intracellular compartments in Arabidopsis root cells, which can be used for example to study endocytosis or secretory trafficking pathways and to compare membrane organization between different genotypes or treatments. While developed for Arabidopsis roots, this method can be used on other tissues, cell types and plant species.
KW  - Arabidopsis
KW  - Endocytosis
KW  - Root
KW  - Compartment
KW  - Confocal analysis
KW  - Image segmentation
KW  - Spot detection
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False