TY  - JOUR
TI  - Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells
AU  - Defez, Roberto 
AU  - Andreozzi, Anna 
AU  - Bianco, Carmen 
VL  - 7
IS  - 2
PY  - 2017
DA  - 2017/01/20
SP  - e2115
C1  - Bio-protocol 2017;7:e2115
DO  - 10.21769/BioProtoc.2115
UR  - https://doi.org/10.21769/BioProtoc.2115
AB  - This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified using a spectrophotometer. This protocol allows the production of reproducible results in a relatively short time for Sinorhizobium meliloti 1021 cells grown both in exponential and stationary phases. The results here presented show that the S. meliloti cells deriving from exponential-phase cultures had increased cell redox potential as compared to the ones deriving from stationary-phase cultures. This means that under exponential growth conditions the S. meliloti cells generate higher amount of reducing equivalents needed for TTC reduction.
KW  - Sinorhizobium meliloti 1021
KW  - Cell redox potential
KW  - 2,3,5-triphenyl-2H-tetrazolium chloride (TTC)
KW  - 1,3,5-Triphenyltetrazolium formazan
KW  - Bacterial cells
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False