TY  - JOUR
TI  - Ex vivo Culture of Fetal Mouse Gastric Epithelial Progenitors
AU  - Vallone, Valeria Fernandez
AU  - Leprovots, Morgane  
AU  - Vassart, Gilbert  
AU  - Garcia, Marie-Isabelle 
VL  - 7
IS  - 1
PY  - 2017
DA  - 2017/01/05
SP  - e2089
C1  - Bio-protocol 2017;7:e2089
DO  - 10.21769/BioProtoc.2089
UR  - https://doi.org/10.21769/BioProtoc.2089
AB  - Isolation and tridimensional culture of murine fetal progenitors from the digestive tract represents a new approach to study the nature and the biological characteristics of these epithelial cells that are present before the onset of the cytodifferentiation process during development. In 2013, Mustata et al. described the isolation of intestinal fetal progenitors growing as spheroids in the ex vivo culture system initially implemented by Sato et al. (2009) to grow adult intestinal stem cells. Noteworthy, fetal-derived spheroids have high self-renewal capacity making easy their indefinite maintenance in culture. Here, we report an adapted protocol for isolation and ex vivo culture and maintenance of fetal epithelial progenitors from distal pre-glandular stomach growing as gastric spheroids (Fernandez Vallone et al., 2016).
KW  - ex vivo
KW  - Spheroids
KW  - Mouse fetal epithelium
KW  - Progenitors
KW  - 3D
KW  - Single cells
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False