TY  - JOUR
TI  - Isolation and Primary Culture of Various Cell Types from Whole Human Endometrial Biopsies
AU  - Barros, Flavio Santos Vasconcelos
AU  - Brosens, Jan Joris
AU  - Brighton, Paul John
VL  - 6
IS  - 22
PY  - 2016
DA  - 2016/11/20
SP  - e2028
C1  - Bio-protocol 2016;6:e2028
DO  - 10.21769/BioProtoc.2028
UR  - https://doi.org/10.21769/BioProtoc.2028
AB  - The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. Cell populations are then isolated through culturing, filtering and magnetic separation using cell-surface antigen markers. Here we provide a comprehensive protocol on how to isolate and culture individual cell types from whole endometrial tissues for use in in vitro experiments.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False