TY  - JOUR
TI  - Determining Efficiency and Selectivity of Lipid Extraction by Perturbing Agents from Model Membranes
AU  - Lafleur, Michel  
AU  - Therrien, Alexandre 
VL  - 6
IS  - 22
PY  - 2016
DA  - 2016/11/20
SP  - e2016
C1  - Bio-protocol 2016;6:e2016
DO  - 10.21769/BioProtoc.2016
UR  - https://doi.org/10.21769/BioProtoc.2016
AB  - Several membrane-perturbing agents extract lipids from membranes and, in some cases, this lipid efflux is lipid specific. In order to gain a better description of this phenomenon and to detail the intermolecular interactions that are involved, a method has been developed to characterize the extent and the specificity of membrane-lipid extraction by perturbing agents. A perturbing agent is incubated with model membranes existing as multilamellar vesicles (MLVs) and subsequently, the remaining MLVs and the small lipid/perturbing agent complexes resulting from the extraction are isolated and analysed to assess the extent and the specificity of the lipid extraction.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False