TY  - JOUR
TI  - In vitro Brainstem-spinal Cord Preparation from Newborn Rat
AU  - Le Gal, Jean-Patrick 
AU  - Nicolosi, Angelo  
AU  - Juvin, Laurent 
AU  - Morin, Didier 
VL  - 6
IS  - 22
PY  - 2016
DA  - 2016/11/20
SP  - e2003
C1  - Bio-protocol 2016;6:e2003
DO  - 10.21769/BioProtoc.2003
UR  - https://doi.org/10.21769/BioProtoc.2003
AB  - The brainstem-spinal cord preparation of newborn rat contains neural networks able to produce motor output in absence of sensory feedback. These neural structures, commonly called central pattern generators (CPGs), are involved in many vital functions such as respiration (Morin and Viala, 2002; Giraudin et al., 2008) or locomotion (Juvin et al., 2005). Here we describe a procedure for the isolation of the brainstem-spinal cord tissue of neonatal rat (0-2 days old). A surgical method under binocular microscope allows the brainstem and the spinal cord to be isolated in vitro and the motor outputs to be recorded. This preparation can then be used for diverse experimental approaches, such as electrophysiology, pharmacology or anatomical studies, and constitutes a useful model to study the interaction between CPGs (Juvin et al., 2007; 2012; Giraudin et al., 2012; Le Gal et al., 2014; 2016).
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False