TY  - JOUR
TI  - Determination of (p)ppGpp Levels During Stringent Response in Streptomyces coelicolor by Thin Layer Chromatography
AU  - Sivapragasam, Smitha  
AU  - Grove, Anne  
VL  - 6
IS  - 21
PY  - 2016
DA  - 2016/11/05
SP  - e1995
C1  - Bio-protocol 2016;6:e1995
DO  - 10.21769/BioProtoc.1995
UR  - https://doi.org/10.21769/BioProtoc.1995
AB  - The stringent response in bacteria is a stress response that is mediated by the signaling molecules guanosine tetraphosphate and pentaphosphate [(p)ppGpp], alarmones that are also directly related to virulence. Therefore, determination of (p)ppGpp levels is crucial for studying the stringent response. The protocol here outlines in a step-wise manner the detection of (p)ppGpp in the bacterium Streptomyces coelicolor during stringent response (Strauch et al., 1991) by thin layer chromatography (TLC). In the example shown here, stringent response is induced by addition of serine hydroxamate, an inhibitor of seryl tRNA synthetase. This protocol was first published in Molecular Microbiology (Sivapragasam and Grove, 2016).
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False