TY  - JOUR
TI  - Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
AU  - Kawasaki , Toshihiro  
AU  - Sakai, Noriyoshi  
VL  - 6
IS  - 21
PY  - 2016
DA  - 2016/11/05
SP  - e1992
C1  - Bio-protocol 2016;6:e1992
DO  - 10.21769/BioProtoc.1992
UR  - https://doi.org/10.21769/BioProtoc.1992
AB  - Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an allogeneic testis and testicular hyperplasia. This protocol can be used to amplify and maintain testicular hyperplasia grafts for several years (Kawasaki et al., 2016). The amplified hyperplasias are likely to be a good source of somatic and germ cells such as Sertoli cells and spermatogonial stem cells.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False