TY  - JOUR
TI  - Detection of Wnt5 in Media Conditioned by Mouse Embryonic Fibroblast
AU  - Flores, Juan 
AU  - Gao, Nan 
VL  - 6
IS  - 20
PY  - 2016
DA  - 2016/10/20
SP  - e1971
C1  - Bio-protocol 2016;6:e1971
DO  - 10.21769/BioProtoc.1971
UR  - https://doi.org/10.21769/BioProtoc.1971
AB  - This protocol describes the procedure of visualizing secreted Wnt5 protein in serum free media via western blotting. This procedure can also be used to visualize other secreted proteins larger than 10,000 daltons. The work presented in this paper visualizes Wnt5 secreted by mouse embryonic fibroblast (MEF), but can be adapted to other cell lines including those transiently transfected by plasmids.
KW  - Wnt5
KW  - Detection
KW  - Conditioned Media
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False