TY  - JOUR
TI  - Aniline Blue and Calcofluor White Staining of Callose and Cellulose in the  Streptophyte Green Algae Zygnema and Klebsormidium
AU  - Herburger, Klaus 
AU  - Holzinger, Andreas 
VL  - 6
IS  - 20
PY  - 2016
DA  - 2016/10/20
SP  - e1969
C1  - Bio-protocol 2016;6:e1969
DO  - 10.21769/BioProtoc.1969
UR  - https://doi.org/10.21769/BioProtoc.1969
AB  - Plant including green algal cells are surrounded by a cell wall, which is a diverse composite of complex polysaccharides and crucial for their function and survival. Here we describe two simple protocols to visualize callose (1→3-β-D-glucose) and cellulose (1→4-β-D-glucose) and related polysaccharides in the cell walls of streptophyte green algae. Untreated or algal cells heated in NaOH are incubated in Calcofluor white (binding to β-glucans including cellulose) or Aniline blue (binding to callose), respectively. Both dyes can be visualized by epifluorescence microscopy.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False