TY  - JOUR
TI  - Evaluation of Burkholderia cepacia Complex Bacteria  Pathogenicity Using Caenorhabditis elegans
AU  - Tedesco, Pietro 
AU  - Di Schiavi, Elia 
AU  - Palma Esposito, Fortunato 
AU  - de Pascale, Donatella 
VL  - 6
IS  - 20
PY  - 2016
DA  - 2016/10/20
SP  - e1964
C1  - Bio-protocol 2016;6:e1964
DO  - 10.21769/BioProtoc.1964
UR  - https://doi.org/10.21769/BioProtoc.1964
AB  - This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia complex (Bcc) strains against the nematode Caenorhabditis elegans. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.
KW  - Burkholderia cepacia complex strains
KW  - Caenorhabditis elegans
KW  - Animal model
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False