TY  - JOUR
TI  - Isolation, Culture, and Staining of Single Myofibers
AU  - Gallot, Yann Simon
AU  - Hindi, Sajedah M.
AU  - Mann, Aman K.
AU  - Kumar, Ashok 
VL  - 6
IS  - 19
PY  - 2016
DA  - 2016/10/05
SP  - e1942
C1  - Bio-protocol 2016;6:e1942
DO  - 10.21769/BioProtoc.1942
UR  - https://doi.org/10.21769/BioProtoc.1942
AB  - Adult skeletal muscle regeneration is orchestrated by a specialized population of adult stem cells called satellite cells, which are localized between the basal lamina and the plasma membrane of myofibers. The process of satellite cell-activation, proliferation, and subsequent differentiation that occurs during muscle regeneration can be recapitulated ex vivo by isolation of single myofibers from skeletal muscles and culturing them under suspension conditions. Here, we describe an improved protocol to evaluate ex vivo satellite cells activation through isolation of single myofibers from extensor digitorum longus (EDL) muscle of mice and culturing and staining of myofiber-associated satellite cells with the markers of self-renewal, proliferation, and differentiation.
KW  - Single myofiber cultures
KW  - Satellite cells
KW  - Muscle injury
KW  - Pax7
KW  - MyoD
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False