TY  - JOUR
TI  - Skin TRITC Painting to Track Dendritic Cells Migrating to the Lymph Nodes
AU  - Hor, Jyh Liang
AU  - Mueller, Scott N.
VL  - 6
IS  - 18
PY  - 2016
DA  - 2016/09/20
SP  - e1939
C1  - Bio-protocol 2016;6:e1939
DO  - 10.21769/BioProtoc.1939
UR  - https://doi.org/10.21769/BioProtoc.1939
AB  - Our protocol describes a simple method that allows tracking of dendritic cells (DC) migration from the flank skin to draining lymph nodes (LN) using a red fluorescent dye tetramethylrhodamine-5-isothiocyanate (TRITC). TRITC is a photostable dye that readily labels cells including DC in the skin and can survive repeated exposure of two-photon laser excitation for prolonged imaging duration. This method can be combined with various fluorescent antibody labels or transgenic mouse strains (such as CD11c-EYFP) to visualize distinct DC populations simultaneously.
KW  - Dendritic cells
KW  - Skin
KW  - Lymph nodes
KW  - Intravital imaging
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False