TY  - JOUR
TI  - Synchronize Human Embryonic Stem Cells at Different Cell Cycle Stage
AU  - Zhu, Hui 
VL  - 2
IS  - 11
PY  - 2012
DA  - 2012/06/05
SP  - e193
C1  - Bio-protocol 2012;2:e193
DO  - 10.21769/BioProtoc.193
UR  - https://doi.org/10.21769/BioProtoc.193
AB  - Pluripotency and the capability for self-renewal are essential characteristics of human embryonic stem cells (hESCs), which hold great potential as a cellular source for tissue replacement. Short cell cycle (15-16 h) compared to somatic cells is another property of hESCs. Efficient synchronization of hESCs at different cell cycle stages is important to elucidate the mechanistic link between cell cycle regulation and cell fate decision. This protocol describes how to establish synchronization of hESCs at different cell cycle stages.
KW  - Cell cycle
KW  - Synchronize
KW  - Human embryonic stem cells
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False