TY  - JOUR
TI  - Bone Marrow Mesenchymal Stem Cells Adhesion Assay
AU  - Yang, Zhigang 
AU  - Xiao, Ran 
VL  - 6
IS  - 15
PY  - 2016
DA  - 2016/08/05
SP  - e1895
C1  - Bio-protocol 2016;6:e1895
DO  - 10.21769/BioProtoc.1895
UR  - https://doi.org/10.21769/BioProtoc.1895
AB  - Mesenchymal stem cells (MSCs) are widespread in adult organisms and involved in tissue maintenance and repair as well as in the regulation of hematopoiesis and immunologic responses. As cell adhesion play important roles in cell interactions and signaling, to thoroughly evaluate the adhesion ability of MSCs is of vital importance to clarify the mechanism of self-renewal, proliferation, activation and migration of MSCs in different microenvironments. Based on the method by Siler et al., 2000, we revised the protocol in order to provide details on how to evaluate the adhesion ability of MSCs from bone marrow (BMSCs) on extracellular matrix (ECM) protein laminins. The current protocol can also be easily translated to MSCs with other treatments or ECMs such as collagens, fibronectin, etc.
KW  - Mesenchymal stem cells
KW  - Extracellular matrix
KW  - Adhesion
KW  - Stem cells
KW  - Laminin
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False