TY  - JOUR
TI  - Extraction and Profiling of Plant Polar Glycerol Lipids
AU  - Liu, Yu  
AU  - Wang, Xuemin  
VL  - 6
IS  - 12
PY  - 2016
DA  - 2016/06/20
SP  - e1849
C1  - Bio-protocol 2016;6:e1849
DO  - 10.21769/BioProtoc.1849
UR  - https://doi.org/10.21769/BioProtoc.1849
AB  - This protocol describes a method to extract total polar glycerol lipids from plant materials, followed by mass spectrometry profiling. Different glycerol lipid classes can be distinguished by their head-groups, which can be profiled automatically and quantitatively by a triple quadrupole mass spectrometry in multiple reaction monitoring (MRM) mode with an autosampler. Comparing with other established methods, such as thin layer chromatography (TLC) separation followed by Gas spectrometry (GC) analysis, this method requires little effort in sample preparation and separation, while the resolution is not limited to general lipid classes but at side chain level. This method was described and used successfully to profile plant lipids changes under freezing stress in Welti et al. (2002).
KW  - Mass spectrometry
KW  - Lipidomics
KW  - Phospholipids
KW  - Glycolipids
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False