TY  - JOUR
TI  - In vitro Fluorescent Matrix Degradation Assay for Entamoeba histolytica
AU  - Emmanuel, Merlyn 
AU  - Datta, Sunando 
VL  - 6
IS  - 6
PY  - 2016
DA  - 2016/03/20
SP  - e1769
C1  - Bio-protocol 2016;6:e1769
DO  - 10.21769/BioProtoc.1769
UR  - https://doi.org/10.21769/BioProtoc.1769
AB  - Fluorescent matrix degradation assay is a popular and widely used assay in the field of invadopodium biology (Artym et al., 2009). Matrix remodeling and degradation can be observed under both physiological and pathological conditions. Cancer cells extensively remodel and degrade the underlying matrix by employing actin-rich protrusive structures called invadosomes. Similar structures are formed by the protozoan parasite Entamoeba histolytica (E. histolytica), upon coming in contact with fibronectin, a major component of the host (extracellular matrix) ECM. Here, we describe a similar assay to measure matrix degradation by Entamoeba histolytica.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False