TY  - JOUR
TI  - Shoot Regenerative Capacity Assays in Arabidopsis and Tobacco
AU  - Zhang, Tian-Qi 
AU  - Wang, Jia-Wei 
VL  - 6
IS  - 5
PY  - 2016
DA  - 2016/03/05
SP  - e1753
C1  - Bio-protocol 2016;6:e1753
DO  - 10.21769/BioProtoc.1753
UR  - https://doi.org/10.21769/BioProtoc.1753
AB  - Plant regeneration refers to a process through which an explant is differentiated to a whole plant under certain conditions. It has been shown that two plant hormones, auxin and cytokinin, play critical roles within this process (Duclercq et al., 2011). Cytokinin induces shoot regeneration, whereas auxin promotes root production. In addition to hormones, recent study has revealed that age cue serves as a common element behind plant cell totipotency (Toledano et al., 2012). Here we present an easy protocol to assess the shoot regenerative capacity of Arabidopsis and tobacco leaves of different ages.
KW  - Shoot regeneration
KW  - Cytokinin
KW  - Auxin
KW  - Plant age
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False