TY  - JOUR
TI  - Infection of Human Hepatocyte-chimeric Mice with HBV and in vivo Treatment with εRNA
AU  - Sato, Seiichi 
AU  - Li, Kai 
AU  - Takaoka, Akinori 
VL  - 6
IS  - 2
PY  - 2016
DA  - 2016/01/20
SP  - e1718
C1  - Bio-protocol 2016;6:e1718
DO  - 10.21769/BioProtoc.1718
UR  - https://doi.org/10.21769/BioProtoc.1718
AB  - Hepatitis B virus (HBV) can cause both acute and chronic disease in human liver with potentially high risk of cirrhosis and liver cancer. The host range of non-human primates susceptible to this virus is limited. Therefore, experimental studies with human hepatocyte-chimeric mice provide an invaluable source of information regarding the biology and pathogenesis of HBV. This section describes the protocol for infection of the human hepatocyte-chimeric mice with HBV. In addition, it has recently been shown that HBV replication can be suppressed by exogenous expression of viral epsilon RNA (εRNA; Sato et al., 2015), which serves as an encapsidation signal (Bartenschlager et al., 1992). Based upon this finding, we also describe the protocol for the liposome-mediated delivery of a plasmid encoding εRNA to liver in these chimeric mice.
KW  - Hepatitis B virus
KW  - Human hepatocyte-chimeric mice
KW  - Infection
KW  - Epsilon RNA
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False