TY  - JOUR
TI  - Identification of RNA-binding Proteins by RNA Ligand-based cDNA Expression Library Screening
AU  - Kim, Min Young
AU  - Lee, Jong Joo
AU  - Kim, Chul Geun
VL  - 6
IS  - 2
PY  - 2016
DA  - 2016/01/20
SP  - e1715
C1  - Bio-protocol 2016;6:e1715
DO  - 10.21769/BioProtoc.1715
UR  - https://doi.org/10.21769/BioProtoc.1715
AB  - We previously reported when a portion of the Requiem (REQ/DPF2) messenger ribonucleic acid (mRNA) 3’ untranslated region (3’UTR), referred to as G8, was overexpressed in K562 cells, β-globin expression was induced, suggesting that the 3’UTR of REQ mRNA plays a physiological role (Kim et al., 2014). To identify trans-acting factors that bind to the REQ 3’UTR, we describe the RNA ligand based cDNA expression library screening method. This protocol could be adapted to detect specific RNA-protein interactions. Following this method, we identified six positive clones in the initial round of screening and four pure clones after sib-screening. This protocol was originally published in Kim et al. (2014).
KW  - RNA-protein interaction
KW  - Phage display
KW  - Requiem (REQ/DPF2)
KW  - CDNA library screening
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False