TY  - JOUR
TI  - Isolation of Lymphocytes from Murine Visceral Adipose Tissue
AU  - Valentić, Sonja 
AU  - Wensveen, Felix M.
AU  - Polić, Bojan 
VL  - 5
IS  - 23
PY  - 2015
DA  - 2015/12/05
SP  - e1669
C1  - Bio-protocol 2015;5:e1669
DO  - 10.21769/BioProtoc.1669
UR  - https://doi.org/10.21769/BioProtoc.1669
AB  - Several studies have shown that the detrimental influence of abdominal obesity on metabolic processes is mediated by the intra-abdominal fat depot. Visceral adipose tissue has been shown to be an independent risk factor for coronary heart disease, hypertension, impaired glucose tolerance and Diabetes Mellitus Type 2 (DM2). Diet-induced obesity in mice, primarily of the C57BL/6J strain, is a commonly used method to study the development of insulin resistance as a model for DM2. The white or visceral adipose tissue (here referred to as VAT), especially the fat around the gonads, is a commonly used organ of study in this model, as it accumulates large numbers of lymphocytes in response to diet-induced obesity. The protocol below describes the isolation of lymphocytes from the stromal vascular fraction (SVF) from VAT.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False