TY  - JOUR
TI  - In vitro and in vivo Limiting Dilution Assay for Colorectal Cancer
AU  - Agro, Lauren 
AU  - O’Brien, Catherine A
VL  - 5
IS  - 22
PY  - 2015
DA  - 2015/11/20
SP  - e1659
C1  - Bio-protocol 2015;5:e1659
DO  - 10.21769/BioProtoc.1659
UR  - https://doi.org/10.21769/BioProtoc.1659
AB  - The in vitro limiting dilution assay is used to determine the colorectal cancer initiating cell (CC-IC) frequency of a CC-IC enriched suspension culture, grown in growth factor enriched serum free media. The in vivo limiting dilution assay is used to determine the colorectal cancer initiating cell frequency of a primary colorectal cancer sample or an established suspension cell line using immunocompromised murine xenograft models. In vitro and in vivo limiting dilution assays (LDAs) can be used to determine the effect of a specific treatment or genetic knockdown strategy on the initiating cell frequency of a population of CC-ICs or colorectal cancer sample, respectively.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False