TY  - JOUR
TI  - Mitochondrial RNA Transcript Analysis Assay of Arabidopsis Leaf Tissues
AU  - Delannoy, Etienne 
AU  - Falcon de Longevialle, Andéol 
AU  - Francs-Small, Catherine Colas des
VL  - 5
IS  - 20
PY  - 2015
DA  - 2015/10/20
SP  - e1620
C1  - Bio-protocol 2015;5:e1620
DO  - 10.21769/BioProtoc.1620
UR  - https://doi.org/10.21769/BioProtoc.1620
AB  - This qPCR-based assay provides an overview of the expression levels of all mitochondrial transcripts (mRNAs and rRNAs) as well as splicing efficiency in Arabidopsis. It was developed before RNAseq techniques were widely used (de Longevialle et al., 2007), but is nevertheless still useful as it is cheaper to run and the analysis is much easier and faster to perform if the aim is only to look at mitochondrial transcripts. For intron-containing mRNAs, the use of primer sets specifically amplifying spliced or unspliced forms allows the evaluation of the splicing efficiency.
KW  - Mitochondrial transcriptome
KW  - Splicing assay
KW  - Quantitative RT-PCR
KW  - Mitochondria
KW  - Arabidopsis thaliana
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False