TY  - JOUR
TI  - Analysis of in vivo Cellulose Biosynthesis in Arabidopsis Cells by Spinning Disk Confocal Microscopy
AU  - Vellosillo, Tamara 
AU  - Yeats, Trevor 
AU  - Sorek, Nadav 
VL  - 5
IS  - 19
PY  - 2015
DA  - 2015/10/05
SP  - e1617
C1  - Bio-protocol 2015;5:e1617
DO  - 10.21769/BioProtoc.1617
UR  - https://doi.org/10.21769/BioProtoc.1617
AB  - Cellulose is a main component of plant cell walls. Tools to analyze cellulose mainly rely on analytical chemistry, which yields information about cellulose amounts and structure, but cannot be applied to intact tissues. Moreover, these methods measure total cellulose and cannot be used to assay cellulose synthesis per se. Live cell imaging of the catalytic subunits of the cellulose synthesis complex (CSC) conjugated to fluorescent proteins is an important tool to understand the dynamics of the cellulose biosynthesis process (Paredez et al., 2006). This method can be used in various genetic backgrounds (Sorek et al., 2014) or with different chemical inhibitors (Brabham and Debolt, 2012). Here we describe in detail the procedure to visualize the movement of CSCs at the plasma membrane. As the movement of CSCs is likely caused by glucan synthesis and extrusion into the cell wall, live cell analysis of CSC velocity provides a method to directly measure cellulose synthesis in vivo.
KW  - Cellulose
KW  - Cell wall
KW  - Cellulose synthesis dynamics
KW  - Live cell imaging
KW  - Arabidopsis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False