TY  - JOUR
TI  - In vitro Studies: Inhibition of Nevirapine Metabolism by Nortriptyline in Hepatic Microsomes
AU  - Usach, Iris 
AU  - Peris, José-Esteban 
VL  - 5
IS  - 19
PY  - 2015
DA  - 2015/10/05
SP  - e1607
C1  - Bio-protocol 2015;5:e1607
DO  - 10.21769/BioProtoc.1607
UR  - https://doi.org/10.21769/BioProtoc.1607
AB  - One of the most prevalent and interfering psychosocial comorbidities of HIV infection is clinical depression (22 to 45%). For this reason, a study of a possible interaction between the nonnucleoside reverse transcriptase inhibitor nevirapine (NVP) and the tricyclic antidepressant nortriptyline (NT) was carried out. In vitro studies with rat and human hepatic microsomes showed a marked inhibition of NVP metabolism by NT being more intense in rat than in human. The extrapolation of these results to humans suggests increased NVP side effects when both drugs are coadministered, but additional in vivo human studies are required to evaluate the clinical implication of this interaction.This protocol describes a technique for detecting and measuring the inhibition of the nevirapine metabolism by nortriptyline in hepatic microsomes.
KW  - Nevirapine
KW  - Nortriptyline
KW  - Metabolism  inhibition
KW  - Drug interaction
KW  - Hepatic microsomes
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False