TY - JOUR TI - Quantitative Evaluation of Competitive Nodulation among Different Sinorhizobium Strains AU - Chun, Chan Lan AU - Nelson, Matthew S. AU - Sadowksy, Michael J. VL - 5 IS - 15 PY - 2015 DA - 2015/08/05 SP - e1555 C1 - Bio-protocol 2015;5:e1555 DO - 10.21769/BioProtoc.1555 UR - https://doi.org/10.21769/BioProtoc.1555 AB - Legumes play a vital role in global food supply because they are uniquely capable of fixing atmospheric nitrogen (N) through symbioses with root and stem nodule bacteria, collectively called the rhizobia. These commonly include bacteria in the genera Rhizobium, Mesorhizobium, Sinorhizobium (Ensifer), and Bradyrhizobium, although other genera of bacteria have now been shown to form root nodule symbioses with several legume species (Weir, 2012). The symbiotic interaction is important for agricultural productivity, especially in less developed countries where nitrogen fertilizer is expensive. However, nodulation ability and competitiveness have practical importance in agricultural production, because the inoculation of efficient rhizobia is often unsuccessful, due to large part to the presence of competitive populations of ineffective indigenous rhizobia in soils (Toro, 1996; Triplett and Sadowsky, 1992). This protocol allows one us to quantitatively evaluate the relative nodulation competitiveness of Sinorhizobium strains. JF - Bio-protocol SN - 2331-8325 PB - Bio-protocol LLC. BIO101 - False