TY  - JOUR
TI  - Nanofluidic Proteomic Immunoassay
AU  - Guo, Huifang 
AU  - Lorenzi, Philip L
AU  - Ding, Zhiyong 
VL  - 5
IS  - 14
PY  - 2015
DA  - 2015/07/20
SP  - e1537
C1  - Bio-protocol 2015;5:e1537
DO  - 10.21769/BioProtoc.1537
UR  - https://doi.org/10.21769/BioProtoc.1537
AB  - Nanofluidic proteomic immunoassay (NIA), consisting of isoelectric focusing followed by sensitive chemiluminescence detection, has the potential to quantitatively characterize protein post-translational modifications that cause shifts in isoelectric point (pI). This protocol details the NIA analysis of protein phosphorylation using AKT as an example.   This protocol can be used for two platforms, NanoPro 1000 and Peggy Sue, from ProteinSimple Company. NanoPro 1000 separates proteins based on charge. Peggy Sue separates proteins based on either charge or size. The platforms can analyze up to 96 samples at a time with lysates from as few as 25 cells per assay. Detailed information of the platforms is available on ProteinSimple’s website (http://www.proteinsimple.com).
KW  - Immunoassay
KW  - Antibody
KW  - AKT
KW  - NIA
KW  - Phosphorylation
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False