TY  - JOUR
TI  - Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
AU  - Massanella, Marta 
AU  - Gianella, Sara 
AU  - Lada, Steven M.
AU  - Richman, Douglas D.
AU  - Strain, Matthew C.
VL  - 5
IS  - 11
PY  - 2015
DA  - 2015/06/05
SP  - e1492
C1  - Bio-protocol 2015;5:e1492
DO  - 10.21769/BioProtoc.1492
UR  - https://doi.org/10.21769/BioProtoc.1492
AB  - Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including EBV) and this HIV reservoir (Gianella et al., 2014). This protocol was designed to simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell (PBMC) aliquot and quantify HIV, CMV and EBV DNA, as well as HIV RNA using droplet digital PCR (ddPCR). For collection and processing of male genital secretions and quantification of HIV RNA and DNA from seven human herpesviruses from seminal plasma, refer to protocol “Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma” (Vargas-Meneses et al., 2015).
KW  - HIV reservoir
KW  - Droplet Digital PCR (ddPCR)
KW  - CMV
KW  - EBV
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False