TY  - JOUR
TI  - Immunoprecipitation of Proteins in Caenorhabditis elegans
AU  - Chan, Kevin K.
AU  - Seetharaman, Ashwin 
AU  - Selman, Guillermo 
AU  - Roy, Peter John
VL  - 5
IS  - 7
PY  - 2015
DA  - 2015/04/05
SP  - e1436
C1  - Bio-protocol 2015;5:e1436
DO  - 10.21769/BioProtoc.1436
UR  - https://doi.org/10.21769/BioProtoc.1436
AB  - Immunoprecipitation (IP) is a biochemical technique to precipitate a protein out of solution using an antigen that can specifically bind to that protein. IP can be performed to isolate and concentrate one particular protein from a sample of thousands of different proteins. IP is also readily performed to pull down interacting proteins of complexes out of solution. This protocol outlines the methods used to IP proteins in whole worm lysates and their preparation for detection on Western blots using denaturing conditions.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False