TY - JOUR TI - Thirty-Second Net Stressor Task in Adult Zebrafish AU - Tran, Steven AU - Gerlai, Robert VL - 5 IS - 5 PY - 2015 DA - 2015/03/05 SP - e1413 C1 - Bio-protocol 2015;5:e1413 DO - 10.21769/BioProtoc.1413 UR - https://doi.org/10.21769/BioProtoc.1413 AB - Zebrafish have become a popular animal model for behavioral neuroscience (Gerlai, 2014). Recent studies have demonstrated that brief experimental handling prior to euthanizing animals can subsequently alter biological measures quantified post-mortem (e.g. cortisol levels) (Ramsay et al., 2009; Tran et al., 2014). Here we provide a detailed protocol for a simple 30-sec net stressor task for adult zebrafish that increases whole-body cortisol levels without altering the levels of whole-brain dopamine, 3, 4-dihydroxyphenylacetic acid, serotonin, and 5-hydroxyindoleacetic acid (Tran et al., 2014). JF - Bio-protocol SN - 2331-8325 PB - Bio-protocol LLC. BIO101 - False