TY  - JOUR
TI  - In vitro Chitin Binding Assay
AU  - Ball, Geneviève 
AU  - Cadoret, Frédéric 
AU  - Voulhoux, Romé 
VL  - 5
IS  - 4
PY  - 2015
DA  - 2015/02/20
SP  - e1401
C1  - Bio-protocol 2015;5:e1401
DO  - 10.21769/BioProtoc.1401
UR  - https://doi.org/10.21769/BioProtoc.1401
AB  - Chitin is polymer of N-acetylglucosamine (GlcNAc) found in the exoskeleton of arthropods and the fungal cell wall. GlcNAc is also implicated in bacterial development, adherence, and signal transduction but can also be used as a carbon source. In vitro chitin binding assay is performed to determine the affinity of a purified protein to the chitin molecule. The principle is based on the co-sedimentation of chitin-binding proteins together with chitin-coated beads.
KW  - Chitin
KW  - N acetylglucosamine
KW  - Chitin beads
KW  - Co-purification
KW  - Affinity
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False