TY  - JOUR
TI  - Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids
AU  - Hsieh, Ming-Hsien 
AU  - Pan, Zhao-Jun 
AU  - Yeh, Hsin-Hung 
AU  - Chen, Hong-Hwa 
VL  - 4
IS  - 24
PY  - 2014
DA  - 2014/12/20
SP  - e1359
C1  - Bio-protocol 2014;4:e1359
DO  - 10.21769/BioProtoc.1359
UR  - https://doi.org/10.21769/BioProtoc.1359
AB  - This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as a silencing vector for floral functional genomics in Phalaenopsis orchids. This protocol is established based on a method created by Lu et al. (2007), and then modified by Hsieh et al. (2013a; 2013b), Lu et al. (2012) successfully engineered a cloning vector (pCymMV-Gateway) in that the target gene fragment is simple to insert and can be manipulated with high efficiency. The silencing vector is inoculated into plants by Agro-inoculation by using Agrobacterium tumefaciens (A. tumefaciens) strain EHA105. Agro-infiltration of leaves for use in VIGS study of orchid flowers is a time saver and produces less damage to flower buds.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False