TY  - JOUR
TI  - RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
AU  - Cecere, Germano 
AU  - Grishok, Alla 
VL  - 4
IS  - 24
PY  - 2014
DA  - 2014/12/20
SP  - e1358
C1  - Bio-protocol 2014;4:e1358
DO  - 10.21769/BioProtoc.1358
UR  - https://doi.org/10.21769/BioProtoc.1358
AB  - The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans) to detect interaction between the nuclear Argonaute CSR-1 (chromosome segregation and RNAi deficient) protein and its target nascent RNAs. We have used a transgenic strain expressing a recombinant long isoform of CSR-1 protein fused with N-terminal 3x FLAG epitope.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False