TY  - JOUR
TI  - Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
AU  - Laoui, Damya 
AU  - Overmeire, Eva Van
AU  - Keirsse, Jiri 
AU  - Movahedi, Kiavash 
AU  - Ginderachter, Jo A Van
VL  - 4
IS  - 22
PY  - 2014
DA  - 2014/11/20
SP  - e1294
C1  - Bio-protocol 2014;4:e1294
DO  - 10.21769/BioProtoc.1294
UR  - https://doi.org/10.21769/BioProtoc.1294
AB  - Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation between their abundance and poor prognosis (Laoui et al., 2011). These cells are able to promote tumor progression via several mechanisms, including induction of angiogenesis, remodeling of the extracellular matrix, stimulation of cancer cell proliferation and metastasis and the inhibition of adaptive immunity (Laoui et al., 2011). Moreover, mononuclear myeloid cells are characterized by plasticity and versatility in response to microenvironmental signals, resulting in different activation states, as illustrated by the presence of distinct functional TAM subsets in tumors (Movahedi et al., 2010; Laoui et al., 2014). Here, we describe a valuable isolation technique for TAM and TADC permitting their molecular and functional characterization.
KW  - Tumor-associated macrophages
KW  - Tumor-associated dendritic cells
KW  - Tumor single cell suspension
KW  - Macrophage purification
KW  - Dendritic cell purification
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False