TY  - JOUR
TI  - Extraction of Total Proteins from Rice Plant
AU  - Lin, Da-Gin 
AU  - Wang, Chang-Sheng 
VL  - 4
IS  - 21
PY  - 2014
DA  - 2014/11/05
SP  - e1277
C1  - Bio-protocol 2014;4:e1277
DO  - 10.21769/BioProtoc.1277
UR  - https://doi.org/10.21769/BioProtoc.1277
AB  - This protocol provides an efficient method for preparation of high-quality proteins from rice leaves and grains. The method involves phenol extraction to separate proteins from the non-protein components such as polysaccharides, lipids and phenolic compounds that are commonly enriched in plant tissues. Following isolation, proteins are precipitated with ammonium acetate/methanol and then solubilized for proteomic analysis. As the protocol is simple, universal, and most importantly compatible with silver staining, it has been applied to our routine protein extraction from rice and many other plant tissues and it even works fine in animal tissues for the requirement of electrophoretic separation.
KW  - Rice
KW  - Protein extraction
KW  - Proteomics
KW  - Two dimentional gel electrophresis
KW  - Staining methods
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False