TY  - JOUR
TI  - Isolation of ILC2 from Mouse Liver
AU  - Mchedlidze, Tamar  
AU  - Wirtz, Stefan  
VL  - 4
IS  - 14
PY  - 2014
DA  - 2014/07/20
SP  - e1179
C1  - Bio-protocol 2014;4:e1179
DO  - 10.21769/BioProtoc.1179
UR  - https://doi.org/10.21769/BioProtoc.1179
AB  - Group 2 innate lymphoid cells (ILC2) are a recently characterized cell population which lacks specific antigen receptors and contributes to immune responses at mucosal surfaces of lung and gut. Recently, we demonstrated that ILC2 expand in the context of chronic liver diseases in mice and contribute to pathology in an IL-33 dependent manner. Here, we describe a protocol to isolate highly purified ILC2 from mouse livers. This procedure provides effective digestion of liver tissue and limits proteolytic degradation of cell surface receptors leading to increased yields of biologically functional cells.  The number of liver resident ILC2 in the steady state is low, however their number dramatically increase upon systemic or local treatment of mice with cytokines such as IL-25 and IL-33. Using minicircle-based expression constructs for these cytokines high numbers of functional ILC2 can be isolated with the protocol provided here.
KW  - Group 2 innate lymphoid cells
KW  - ILC2
KW  - Inflammation
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False