TY  - JOUR
TI  - Construction of Deletion-knockout Mutant Fowlpox Virus (FWPV)
AU  - Laidlaw, Stephen  M.
AU  - Skinner, Michael  A.
VL  - 4
IS  - 10
PY  - 2014
DA  - 2014/05/20
SP  - e1126
C1  - Bio-protocol 2014;4:e1126
DO  - 10.21769/BioProtoc.1126
UR  - https://doi.org/10.21769/BioProtoc.1126
AB  - The construction of deletion-knockout poxviruses is a useful approach to determining the function of specific virus genes. This protocol is an adaptation of the transient dominant knockout selection protocol published by Falkner and Moss (1990) for use with vaccinia virus. The protocol makes use of the dominant selectable marker Escherichia coli guanine phosphoribosyltransferase (gpt) gene (Mulligan and Berg, 1981), under the control of an early/late poxvirus promoter. The deletion viruses that are produced no longer contain a selectable marker, which may be preferable for the production of vaccines.
KW  - Knockout
KW  - Fowlpox
KW  - Deletion
KW  - Poxvirus
KW  - Transient dominant
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False