TY  - JOUR
TI  - Extraction and Measurement the Activities of Cytosolic Phosphoenolpyruvate Carboxykinase (PEPCK) and Plastidic NADP-dependent Malic Enzyme (ME) on Tomato (Solanum lycopersicum)
AU  - Osorio, Sonia  
AU  - Vallarino, José  G.
AU  - Szecowka, Marek  
AU  - Ufaz, Shai  
AU  - Tzin, Vered  
AU  - Angelovici, Ruthie  
AU  - Galili, Gad  
AU  - Fernie, Alisdair  R.
VL  - 4
IS  - 9
PY  - 2014
DA  - 2014/05/05
SP  - e1122
C1  - Bio-protocol 2014;4:e1122
DO  - 10.21769/BioProtoc.1122
UR  - https://doi.org/10.21769/BioProtoc.1122
AB  - A recent study demonstrated that cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and NADP-malic enzyme (NADP-ME) have an important role in malate metabolism during fruit ripening (Osorio et al., 2013). PEPCK catalyze the ATP-dependent decarboxylation of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) and NADP-ME, the reversible conversion of malate and pyruvate. Here, we present the detailed protocols to measure PEPCK activity in carboxylation direction by following oxidation of NADH and to measure NADP-ME activity based upon the reduction of NADP+.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False