TY  - JOUR
TI  - The BhbA Enzyme Assay
AU  - Chen, Kai  
AU  - Jiang, Jiandong  
VL  - 4
IS  - 8
PY  - 2014
DA  - 2014/04/20
SP  - e1111
C1  - Bio-protocol 2014;4:e1111
DO  - 10.21769/BioProtoc.1111
UR  - https://doi.org/10.21769/BioProtoc.1111
AB  - Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp. 7D-2, which was isolated from a bromoxynil octanoate-contaminated soil sample collected in Jiangsu, China. BhbA catalyzes the reductive dehalogenation of bromoxynil and its derivative 3,5-dibromo-4-hydroxybenzoate under aerobic conditions. BhbA is membrane-associated and found to have the key features of anaerobic respiratory reductive dehalogenases. This protocol describes the method for enzyme analysis of the aerobic reductive dehalogenase (BhbA) in the membrane fraction.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False