TY  - JOUR
TI  - Isolation of Cells from Human Intestinal Tissue
AU  - Uronen-Hansson, Heli  
AU  - Persson, Emma  
AU  - Nilsson, Petra  
AU  - Agace, William  
VL  - 4
IS  - 7
PY  - 2014
DA  - 2014/04/05
SP  - e1092
C1  - Bio-protocol 2014;4:e1092
DO  - 10.21769/BioProtoc.1092
UR  - https://doi.org/10.21769/BioProtoc.1092
AB  - The intestinal lamina propria contains a dense network of T cells, dendritic cells (DCs) and macrophages, which play an important role in local innate and adaptive immune responses. We have recently identified distinct subsets of DCs (Persson et al., 2013) and macrophages (Bain et al., 2013) in the human intestine. In addition, we have studied T cells in healthy and diseased intestine. Here, we describe two methods for isolating these cell populations: 1) enzymatic treatment and 2) migration based isolation. The enzymatic method can be used to isolate T cells, DC and macrophages, whereas the migration based ‘walk-out’ protocol is suitable for DC isolation, as these cells migrate out from the tissues.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False