TY  - JOUR
TI  - Isolation and Culture of Neurospheres for the Study of Pathogenesis of Prion Disease
AU  - Iwamaru, Yoshifumi  
AU  - Takenouchi, Takato  
AU  - Kitani, Hiroshi  
VL  - 4
IS  - 6
PY  - 2014
DA  - 2014/03/20
SP  - e1081
C1  - Bio-protocol 2014;4:e1081
DO  - 10.21769/BioProtoc.1081
UR  - https://doi.org/10.21769/BioProtoc.1081
AB  - Neurosphere contains neural stem cells that are capable of self-renewal and multilineage differentiation including neurons, astrocytes, and oligodendrocytes (Gage, 2000). Cell culture model using differentiated neurosphere cultures are suggested to be a valuable tool for studying the pathogenesis of prion disease at the cellular level (Iwamaru et al., 2013). This protocol describes the procedure for a culture of whole brain-derived neurospheres from newborn mouse brains. Neurosphere formation steadily occurs within a week from the cultures of neonatal whole brains and these cells have stem cell properties.
KW  - Neural stem cell
KW  - Isolation and culture
KW  - Neurosphere
KW  - Neonatal mouse brain
KW  - Prion protein
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False